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Pack your carry-on with essentials first -- medication, identification, a copy of your itinerary, and everything you'll need for the first 24 hours at your destination, just in case something happens to your checked bag. If you're bringing important business materials, such as a presentation or portfolio, bring them in your carry-on, too, as they're less likely to go astray.
If you're bringing a laptop or cell phone, be sure to have chargers, adapters and other accessories available in your carry-on. Also consider a pair of noise-canceling headphones if you plan to spend time concentrating on work (or even just reading a good book) during the flight.